A Practical Guide to Protein and Peptide Purification for Microsequencing

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. The highlights of the Second Edition include: mass spectrophotometric characterization of proteins and peptides; N-terminal sequencing of proteins with blocked N termini; Internal amino acid sequence analysis after protease digestion in-gel and on-blot; improved microscale peptide purification methods; computer analysis of protein sequences; new protocols tested and refined through everyday use in authors' laboratories; and, updated reference chapter covering all aspects of protein microsequencing.