Microarray Technology Through Applications

Microarray Technology Through Applications provides the reader with an understanding, from an applications perspective, of the diverse range of concepts required to master the experimental and data analysis aspects of microarray technology.

The book is divided into three sections. Section 1 is a concise introduction to the technology and provide the theoretical background to understanding the subsequent sections. It also includes an independent scientific review of the products available from the five main manufacturers of microarray equipment. Section 2 is a series of chapters describing case studies representative of the most general and important applications of microarray technology. The case studies are written by experts in the field and describe prototypic projects, indicating how to generalize the approach to similar studies. The last section of the book contains detailed step-by-step protocols describing the specific experimental and data analysis protocols mentioned in the case study section

1. Introduction to Microarray Technology 2. Immunoprecipitation with Microarrays to Determine the Genome-Wide Binding Profile of a DNA-Associated Protein 3. Array-Based Comparative Genomic Hybridization (Array CGH) as a Tool for Solving Practical Biological and Medical Questions 4. Use of SNPs Arrays : Design, Tools and Applications 5. "In vitro" Analysis of Gene Expression 6. The Analysis of Cellular Transcriptional Response at the Genome Level : Two case Studies with relevance to Bacterial Pathogenesis 7. Functional Annotation of Microarray Experiments 8. Microarray Technology in Agricultural Research 9. Protein Microarrays Protocol 1. Microarray Printing - Standard Operating Procedure for QIAGEN OPERON Oligonucleotide Arrays Protocol 2. "E. coli "RNA Extraction Protocol 3. Indirect Labelling of First-strand cDNA Probes Protocol 4. Pre-Hybridization and Washing Protocol for UltraGAP Slides Protocol 5. ChIP Procedure Protocol 6. Array CGH Protocol 7. "In vitro" Analysis of Gene Expression Using Run-off Microarray Analysis of Gene Expression (ROMA) Appendix 1. Notes on Printing Glass DNA Microarray Slides Appendix 2. Comparative Genomics : The Nature of CGH Analysis and Data Interpretation Appendix 3. Useful Links Appendix 4. Table 7