Plant Molecular Biology Manual, Sixth Supplement (not avail seperately)

A comprehensive manual dealing with the molecular aspects of plant molecular biology, which is designed both as a teaching aid for advanced laboratory courses and as a guide for laboratory research. Subjects covered include the introduction of genes into plants, assays of gene activity, plant nucleic acid extraction and characterization procedures, and the use of selectable and screenable markers in transgenic plants. Special emphasis is placed on the use of Agrobacterium tumefaciens to introduce genes into plants, but other gene introduction techniques are also presented. Each chapter, written by experts in the field, includes a discussion of the background to each technique, detailed protocols, media and solution formulas, and an extensive bibliography. The manual is published in a looseleaf format which is useful for laboratory practice and also allows updating and expansion to be accomplished with ease. The manual has been updated by the publication of the Sixth Supplement.

Contents for Supplement 5: Detection of DNA sequence variation for genome analysis; Pulsed field gel electrophoresis; Physical mapping by random clone fingerprint analysis; Construction and application of a YAC library. Supplement 6: 1. Isolation of DNA-binding proteins by direct screening of cDNA libraries with labelled binding sites; K.B. Singh. 2. In vitro footprinting by LMPCR procedure; P. Brignon, C. Gigot, N. Chaubet. 3. Random Amplified Polymorphic DNA (RAPD) Markers; A. Rafalski, S. Tingey, J.G.K. Williams. 4. Update chapter "Agrobacterium Molecular Genetics"; T. Mozo, P.J.J. Hooykaas.